Coding

Part:BBa_I748002:Design

Designed by: Kurt Whittemore   Group: iGEM07_Southern_Utah   (2007-10-26)


Putative Nitrilase Transcriptional Regulator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 975
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 129
    Illegal NgoMIV site found at 148
    Illegal AgeI site found at 712
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We isolated this sequence using a PCR reaction.

Source

This part comes from the Pseudomonas fluorescens PfO-1 (Accession NC_007492). It codes for a transcriptional regulator protein belonging to the AraC family (protein_id: YP_349418.1 and GeneID: 3715135). We isolated the sequence from 300 base pairs upstream of the gene in the genome to 30 base pairs downstream.

References